Skip to main content
Fig. 1 | Cell & Bioscience

Fig. 1

From: PIAS1 potentiates the anti-EBV activity of SAMHD1 through SUMOylation

Fig. 1

PIAS family proteins interact with SAMHD1. A Schematic representation of V5-Tagged PIAS family proteins. SAP (SAF-A/B, Acinus, and PIAS): DNA and protein binding domain; PINIT: nuclear localization motif; RING finger: E3 ligase domain for protein SUMOylation; SIM: SUMO interacting motif; S/T Rich: variable Ser/Thr rich region. B PIAS1, PIAS2, and PIAS4 interact with SAMHD1. WB analysis showing Co-IP of SAMHD1 with PIAS proteins using 293 T cells transfected with SAMHD1 and individual PIAS proteins as indicated. Input, 2% whole-cell lysate used for IP. Left arrow denotes modified SAMHD1 in the PIAS1 and SAMHD1 co-transfection group. Anti-SUMO2/3 antibody was used to show a SUMOylated band as indicated (right arrow). C PIAS1 interacts with SAMHD1 in vivo. Endogenous PIAS1 was immunoprecipitated by anti-PIAS1 antibody or rabbit IgG control using cell lysate from 293 T cells. The Co-IP of SAMHD1 was examined by WB analysis. The positions of PIAS1 and SAMHD1 were labeled by arrowheads. Arrow denotes modified SAMHD1. D PIAS1 promotes SAMHD1 SUMOylation. Endogenous SAMHD1 was immunoprecipitated from cells transfected with vector control or PIAS1. WB was performed using anti-SUMO2/3 or other antibodies as indicated. Bracket denotes SUMOylated SAMHD1

Back to article page