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Fig. 2 | Cell & Bioscience

Fig. 2

From: Discovery of chemerin as the new chemoattractant of human mesenchymal stem cells

Fig. 2Fig. 2

Chemerin induced phosphorylation of JNK1/2, ERK1/2, and p38 and increased the expression of migration-related genes in hBM-MSCs. A Western blot of phosphorylated JNK1/2, p38, and ERK1/2 in hBM-MSCs after chemerin treatment. Protein expression was quantified using the Image J software. Expression was normalized to that of GAPDH (N > 3). B RT-qPCR of CD44, ITGA4, and MMP-2 after chemerin treatment. mRNA levels were observed 24 h after the chemerin treatment of BM-MSCs. C Western blot analysis of CD44, ITGA4, and MMP-2 of hBM-MSCs after chemerin treatment. Protein expression was quantified using Image J and normalized to GAPDH level. D Venn diagram of common transcription factors, CD44, ITGA4, and MMP2. Transcription of each gene was found using GP miner and 26 genes were found to encode common transcription factors. Among these transcription factors, five were involved in transmigration. GP miner (http://gpminer.mbc.nctu.edu.tw/). E RT-qPCR of transcription factors in naïve and chemerin-treated hBM-MSCs. The data shown were reproducible results from three independent experiments

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