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Fig. 8 | Cell & Bioscience

Fig. 8

From: ADP-ribosyltransferase PARP11 suppresses Zika virus in synergy with PARP12

Fig. 8

PARP11 interacts and co-localizes with PARP12. a Western blotting analysis of lysates immunoprecipitated for PARP12 from HEK293T cells co-transfected with Flag-PARP11 and GFP-PARP12 plasmids. b Confocal microscopy analysis of Vero cells that were co-transfected with EGFP-PARP12, RFP-PARP11 and vector control plasmids. Scale bars, 5 μM. c Western blotting analysis of lysates immunoprecipitated for PARP12 from HEK293T cells co-transfected with HA-PARP12 and EGFP-PARP11 WWE or EGFP-PARP11 PARP plasmids. d Sketch map of the functional regions of full length (FL) PARP12 and mutant constructs. e Western blotting analysis of lysates immunoprecipitated for PARP11 from HEK293T cells co-transfected with HA-PARP12 ZnF, HA-PARP12 WWE, HA-PARP12 PARP and YFP-PARP11 plasmids. f Western blotting analysis of lysates immunoprecipitated for NS1 from HEK293T cells co-transfected with HA-PARP12 ZnF, HA-PARP12 WWE, HA-PARP12 PARP and His-NS1 plasmids. Western blotting (a, c, e and f) and confocal images (b) were presentative of three independent experiments

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