Fig. 7

PARP11 suppresses ZIKV mostly dependent on PARP12. a–c PARP11-overexpressing or control vector-transfected WT and PARP12^−/− A549 cells were infected with ZIKV. Viral accumulation after 48 h in the culture supernatants (a and b) and cell lysates (c) were measured by plaque assay (a) or qRT-PCR (b and c). PFU, plaque-forming unites. d Western blotting analysis of cell lysates of cell lysates from WT and PARP12^−/− HEK293T cells transfected with His-NS1 or NS3 and decreasing amounts of YFP-PARP11 plasmids (500, 300, 100, 0 ng). e Densitometry analysis of the data in (d). f Western blotting analysis of cell lysates of cell lysates from WT and PARP12^−/− HEK293T cells transfected with His-NS1 or NS3 and decreasing amounts of YFP-PARP11 enzyme lost mutant plasmids (500, 300, 100, 0 ng). g Densitometry analysis of the data in (f). qRT-PCR, viral titers and densitometry analysis data (a–c, e and g) are mean ± SEM from three independent experiments. Western blotting results (d and f) are presentative of three independent experiments. **P < 0.01, ***P < 0.001 and ****P < 0.0001 by Student’s t test