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Fig. 6 | Cell & Bioscience

Fig. 6

From: ADP-ribosyltransferase PARP11 suppresses Zika virus in synergy with PARP12

Fig. 6

PARP11 promotes PARP12-mediated NS1 and NS3 degradation independent on its PARP enzyme activity. a Western blotting analysis of cell lysates from WT, PARP11−/− and PARP12−/− HEK293T transfected with His-NS1 or NS3 and increasing amounts of HA-PARP12 plasmids (0, 250, 500 ng). b Densitometry analysis of the data in (a). c Western blotting analysis of cell lysates from WT, PARP11−/− and PARP12−/− HEK293T transfected with HA-PARP12, YFP-PARP11 and His-NS1 or His-NS3 plasmids. d Densitometry analysis of the data in (c). e Western blotting analysis of cell lysates from WT, PARP11−/−, PARP12−/− and PARP11−/−PARP12−/− HEK293T transfected with HA-PARP12, His-NS1/NS3 and FL PARP11 and mutant constructs plasmids. FL: full length; WWE: WWE domain; PARP: Poly (ADP-ribose) polymerase domain. PARP PM: PARP domain with point mutation to inactivate PARP enzyme activity. PM: PARP11 full length protein with point mutation to inactivate PARP enzyme activity. f Densitometry analysis of the data in (e). Western blotting results (a, c and e) are presentative of three independent experiments. Densitometry analysis data (b, d and f) are mean ± SEM pooled from three independent experiments. ns, not significant, *P < 0.05, **P < 0.01, ***P < 0.001, and ***P < 0.0001 by Student’s t test.

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