Fig. 5

PARP11 promotes PARP12-mediated ZIKV NS1 and NS3 protein degradation. a Western blotting analysis of cell lysates from WT, PARP11^−/−, PARP12^−/− and PARP11^−/−PARP12^−/− HEK293T transfected with HA-PARP12, YFP-PARP11, Flag-PARP13 and His-NS1 or His-NS3 plasmids as indicated. b Densitometry analysis of the data in (a). c Western blotting analysis of expression of NS1 and NS3 proteins from PARP11-overexpressing or control vector-transfected A549 cells infected with ZIKV for 48 h. d Densitometry analysis of the data in (c). e Western blotting analysis of expression of NS1 and NS3 proteins from WT or PARP11^−/− A549 cells infected with ZIKV for 48 h. f Densitometry analysis of the data in (e). Western blotting results (a, c and e) are presentative of three independent experiments. Densitometry analysis data (b, d and f) are mean ± SEM pooled from three independent experiments. ns, not significant, *P < 0.05, **P < 0.01, and ***P < 0.001 by Student’s t test