Fig. 4

PARP11 suppresses ZIKV independent on its PARP enzyme activity. a Sketch map of the functional regions of full length (FL) PARP11 and mutant constructs. FL: full length; WWE: WWE domain; PARP: Poly (ADP-ribose) polymerase domain. PM: Point mutation to inactivate PARP enzyme activity. b Western blotting analysis of lysates from HEK293T cells transfected with the plasmids encoding GFP-tagged FL and mutant PARP11 constructs. c HeLa cells were transfected with FL PARP11 and mutant constructs and infected with ZIKV 24 h after transfection. 48 h post infection, ZIKV titers in HeLa cells culture supernatant were measured by plaque assay. Western blotting results (b) are presentative of three independent experiments. Viral titers (c) are mean ± SEM from three independent experiments. ***P < 0.001 by Student’s t test