Fig.1

The expression of PARP11 can be induced by IFN and ZIKV infection. a, b qRT-PCR analysis of PARP11 expression in WT and IFNAR1^−/− HEK293T (a) or A549 (b) cells treated with recombinant human IFN-α (1000 U/mL), IFN-β (20 ng/mL) and control for 24 h. c, d Western blotting analysis of PARP11 expression in WT and IFNAR1^−/− HEK293T (c) or A549 (d) cells treated with recombinant human IFN-α (1000 U/mL), IFN-β (20 ng/mL) and control for 12 and 24 h. e Densitometry analysis of the data in (c). f Densitometry analysis of the data in (d). g qRT-PCR analysis of PARP11 expression in WT and IFNAR1^−/− A549 cells infected with ZIKV for the indicated times. h Western blotting analysis of PARP11 expression in WT and IFNAR1^−/− A549 cells infected with ZIKV for the indicated times. i Densitometry analysis of the data in (h). qRT-PCR (a, b and g) and densitometry (e, f and i) data are mean ± SEM from three independent experiments. Western blotting results (c, d and h) are representative images of three independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001 and ****P < 0.0001 by Student’s t test