Fig. 8
Intestinal epithelium-specific deletion of PRMT1 accelerates the epithelial migration along the crypt-vilus axis. The proximal (A) or distal (B) small intestine of 8-week-old wild type (PRMT1fl/flVil-Cre−/−) or PRMT1 knockout (PRMT1fl/flVil-Cre+/−) mice were sectioned at indicated times after EdU injection. Note that in both proximal and distal intestine, essentially all EdU-labeled cells were located in the crypts at 2 h after EdU injection in both wild type and knockout animals. 24 h after EdU injection, EdU-labeled cells migrated up to the lower middle of villus, with the faster migration in the knockout mice. 48 h after EdU injection, EdU-labeled cells migrated to the middle (for proximal intestine) or near the top (for distal intestine) of villus in wild type animals but in the knockout animals, most of the labeled cells were lost, suggesting that they had migrated to the very top of the villus and undergone apoptosis