Fig. 1

Generation and confirmation of intestinal epithelium-specific PRMT1 conditional knockout mice. A Schematic diagram of the wild-type PRMT1 allele with LoxP sites (white arrowheads) flanking exons 5 and 6, and an FRT-flanked β-galactosidase/neomycin (β-gal/neo) cassette, which can be removed by crossing the PRMT1^LoxP mice with Flp (Flipase)-expressing mice to produce mice containing PRMT1^fl allele. The resulting PRMT1^fl-containing mice can be crossed with mice expressing the Cre recombinase under the control of the Villin promoter to remove the floxed exons 5 and 6, thus knocking out this PRMT1 allele. B PCR-genotyping of genomic DNA extracted from tail biopsies. Homozygous PRMT1^fl/fl mice were crossed with mice that were heterozygous in Villin Cre and floxed PRMT1 (PRMT1^fl/+Cre^+/−). The resulting animals as well as wild type (WT) were genotyped by PCR with a PRMT1 primer combination that distinguishes WT, PRMT1^fl/fl and PRMT1^fl/+ genotypes, which produced bands in agarose gel of 377 bp for PRMT1 floxed allele and 277 bp for WT PRMT1 allele., respectively. The presence or absence of Cre was also analyzed by PCR with a specific primer pair to produce a band of about 1000 bp