Fig. 2

SARS-CoV-2 Spike protein mediates cell fusion in transduced 293T cells. A) Representative images of cell–cell fusion in co-cultures of either S-WT-293T and hACE2-293T cells with EVOS FL color image systems (top panel) or confocal microscope (bottom panel) (DAPI, blue; S-WT-EGFP or S-Δ19-EGFP, green; hACE2-mCherry, red). Blue circle indicates the cell fusion in co-culture of S-WT-293T and hACE2-293T. B Representative images of cell–cell fusion in different co-cultures of S-Δ19-293T, and hACE2-293T cells with EVOS FL color image systems (top panel) or confocal microscope (bottom panel) (DAPI, blue; S-WT-EGFP or S-Δ19-EGFP, green; hACE2-mCherry, red). Red circles indicate the cell fusion in co-culture of S-Δ19-293T and hACE2-293T. Scale bar equals 40 µm