Fig. 6

JAZF1-Het mice fed with HFD showed a lower adipose development. Eight-week-old JAZF1-Het-HFD (Het-HFD) and JAZF1-Cont-HFD (Cont-HFD) mice were fed with HFD for 8 weeks. a The body weight gaining accumulation during the 8 weeks of HFD feeding in both the JAZF1-Het-HFD and JAZF1-Cont-HFD mice (n = 8). b The morphology of eWAT and liver tissue in JAZF1-Het-HFD and JAZF1-Cont-HFD after 8 weeks of HFD feeding. c, d The percentage of eWAT and liver organ weight was measured to the total body weight (n = 7). e HH&E staining of eWAT and scWAT in both the JAZF1-Het-HFD and JAZF1-Cont-HFD mice. Scale bar, 100 µm. f Quantification of eWAT and scWAT adipocyte diameters frequency in JAZF1-Het-HFD and JAZF1-Cont-HFD mice (right panel). Mean adipocyte diameter was also quantified by calculation of each single adipocyte diameter (left panel). The diameters in eWAT and scWAT were quantified in eight random images from each mouse (n = 7). g–i Relative mRNA expression of the adipogenic marker (PPARγ2, C/EBPα, and FABP4) in eWAT, scWAT, and BAT from both the JAZF1-Het-HFD and the JAZF1-Cont-HFD mice (n = 6). All data are presented as mean ± SEM. *p < 0.05, **p < 0.01, and ***p < 0.001. eWAT epididymal white adipose tissue, scWAT subcutaneous adipose tissue, BAT brown adipose tissue