Fig. 4

JAZF1-Het mice show impaired glucose homeostasis. JAZF1-Het (Het) and JAZF1-Cont (Cont) mice were fed with ND for 8 weeks starting at 8-week-old. After 8 weeks of ND feeding. a–c Relative mRNA expression of adipogenic markers (PPARγ2, C/EBPα, and FABP4) in eWAT, scWAT, and BAT from JAZF1-Het and JAZF1-Cont mice fed with ND for 8 weeks (n = 6). d, e GTT blood glucose levels were measured in male and female JAZF1-Het and JAZF1-Cont mice after injecting the glucose (1.5 g/kg body weight [BW], n = 8). f, g ITT blood glucose levels in male and female JAZF1-Het and JAZF1-Cont mice after injecting the insulin (2 U/kg BW, n = 8). h Area under the curve (AUC) of the glucose levels in male and female JAZF1-Het and JAZF1-Cont mice (n = 8 for each group), as determined by GTT and ITT glucose levels. The data is shown by percent (%) of control mice AUC glucose levels. i Serum insulin levels in 6 h fasted male and female JAZF1-Het and JAZF1-Cont mice after 8 weeks of ND feeding. (n = 6 or 7 for each group). j Western blotting of insulin-stimulated (2 U/Kg BW) AKT phosphorylation in the eWAT and liver lysates from both the JAZF1-Het and the JAZF1-Cont mice fed with ND for 8 weeks. All data are presented as mean ± SEM. *p < 0.05 and **p < 0.01. GTT glucose tolerance test, ITT insulin tolerance test, eWAT epididymal white adipose tissue, scWAT subcutaneous adipose tissue, BAT brown adipose tissue