Fig. 2

JAZF1 deficiency show impaired adipocyte differentiation in vitro. MEFs were isolated from E13.5 JAZF1-KO (KO), JAZF1-Het (Het), and JAZF1-Cont (Cont) embryos. a JAZF1 mRNA expressions were confirmed by semiquantitative PCR in isolated MEFs. b JAZF1-KO, JAZF1-Het, and JAZF1-Cont MEFs were induced adipocyte differentiation. Twelve days after induction of adipocyte differentiation, cells were stained with Oil red O. The staining was quantified spectrophotometrically at 550 nm. Results are representative of three independent experiments. c Relative mRNA expression of adipogenic markers after adipocyte differentiation (Day 12) in JAZF1-KO, JAZF1-Het, and JAZF1-Cont MEFs. Results are representative of three independent experiments. qRT-PCR time-course analyses of the relative d JAZF1, e PPARγ2, f C/EBPα, and g FABP4 and h C/EBPβ mRNA expressions during early (0–2 days) and terminal (2–8 days) adipocyte differentiation in JAZF1-KO and JAZF1-Cont MEFs. Results are representative of three independent experiments. i Western blot analyses of JAZF1, PPARγ, and C/EBPβ protein expressions, during adipocyte differentiation, in JAZF1-KO and JAZF1-Cont MEFs. All data are presented as mean ± SEM. *p < 0.05, **p < 0.01, and ***p < 0.001. MEFs mouse embryonic fibroblasts