Fig. 1

JAZF1 expression is regulated during 3T3-L1 preadipocyte differentiation. Quantitative real time polymerase chain reaction (qRT-PCR) time-course analyses of the relative a JAZF1, b PPARγ2, c C/EBPα, and d C/EBPβ mRNA expressions during early (0–2 days) and terminal (2–8 days) adipocyte differentiation in 3T3-L1. Results are representative of three independent experiments. e Western blot time-course analyses of JAZF1, PPARγ, and C/EBPβ protein expressions during early and terminal adipocyte differentiation in 3T3-L1. f JAZF1 protein (upper panel) and mRNA (bottom panel) expressions in SVFs and mature adipocytes from fractionated eWAT in wild-type C57BL/6J mice. Results (mRNA expression) are representative of three independent experiments. All data are presented as mean ± SEM. *p < 0.05, **p < 0.01, and ***p < 0.001. SVFs stromal vascular fractions