Table 1 Primer sequences used in real-time PCR
From: Rev-erbα exacerbates hepatic steatosis in alcoholic liver diseases through regulating autophagy
Genes | Forward primer (5′–3′) | Reverse primer (5′–3′) |
|---|---|---|
(Human) Rev-erbα | TTGAGTCAAGGTCCAGT TTGAATG | GGAGTCCAGGGTCGTC ATGT |
(Human) Rev-erbβ | GTCAAGGTCCAGTTTGA ATG | GCGAGATCACCATTCTT GGG |
(Human) Pparα | GCACCTGGAGGTATCG TCGAT | CATGGGACCCTTATCA ATCCTAATC |
(Human) Srebp1c | GGGTCAGTTGTCCCTTC TCA | TGAGACGTGCCAGACT TCTT |
(Human) BMAL1 | AGCTGCCTCGTCGCAAT T | CCGTTCACTGGTTGTGG AACT |
(Human) β-actin | CCCTGGAGAAGAGCTA CGAG | TGCTAGGAGCCAGAGC AGTA |
(Mouse) Rev-erbα | CCCAACGACAACAACC TTTT | CCCTGGCGTAGACCAT TCAG |
(Mouse) Rev-erbβ | GGTTAGGTTTGTGAGTG TCCACAGC | GGAAGTGCTCCAACAA GGTAGTGCA |
(Mouse) Pparα | AGCTGGTGTAGCAAGT GT | TCTGCTTTCAGTTTTGC TTT |
(Mouse) Srebp1c | ACACAGCAACCAGAAA CTCAAG | AGTGTGTCCTCCACCTC AGTCT |
(Mouse) β-actin | AGTGTGACGTTGACATC CG | TGCTAGGAGCCAGAGC AGTA |