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Fig. 6 | Cell & Bioscience

Fig. 6

From: Rev-erbα exacerbates hepatic steatosis in alcoholic liver diseases through regulating autophagy

Fig. 6

Rev-erbα inhibits the activity of autophagy through regulating Bmal1. A Western blot analysis of Bmal1 in nucleus and cytoplasm in EtOH-treated L-02 cells. Bar represents the mean ± SEM. Significance *P < 0.05, **P < 0.01 vs. EtOH-fed group or EtOH group. B Western blot analysis of Bmal1 in EtOH-treated L-02 cells transfected with Rev-erbα shRNA. Bar represents the mean ± SEM. Significance *P < 0.05, **P < 0.01 vs. control group, #P < 0.05, ##P < 0.01 vs. control shRNA group. C Lysosome staining by using Lyso Tracter Green DND-26 (50 nM) in EtOH-treated L-02 cells transfected with Bmal1 siRNA and Rev-erbα shRNA (n = 3) (scale bars = 40 μm). D Western blot analysis of Lc3II/I, P62, Pparα and Srebp1c in EtOH-treated L-02 cells transfected with Bmal1 siRNA and Rev-erbα shRNA. Bar represents the mean ± SEM. Significance *P < 0.05, **P < 0.01 vs. EtOH + control shRNA group, #P < 0.05, ##P < 0.01 vs. EtOH + Rev-erbα shRNA + control siRNA group

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