Fig. 2

Rev-erbα was up-regulated in EtOH-treated L-02 cells and mediated liver steatosis in vitro. L-02 cells were treated with 150 mM EtOH for 48 h. A Western blot analysis of Rev-erbα expression in Control and EtOH group. B qRT-PCR analysis of Rev-erbα and Rev-erbβ in Control and EtOH group. C Immunofluorescence analysis of nuclear localization of Rev-erbα in Control and EtOH group (scale bar = 40 μm). D Western blot analysis of Rev-erbα in nucleus and cytoplasm in control and EtOH group. L-02 cells were treated with or without 10 μM GSK4112 for 24 h. E, F ORO staining and TG content analysis in control and GSK4112 group (scale bar = 100 μm). G Western blot analysis of Pparα and Srebp1c in control and GSK4112 group (n = 3). Bar represents the mean ± SEM. Significance *P < 0.05, **P < 0.01 vs. control group, ^#P < 0.05, ^##P < 0.01 vs. Rev-erbα in control group