Fig. 1

Rev-erbα was up-regulated in the liver of EtOH-fed mice in vivo. C57 male mice weigh 20 kg or more were fed EtOH liquid diet or the Control diet for 16 days. A Representative image of liver and histological assessment of hepatic pathologic alterations by H&E and ORO staining in alcoholic fatty liver mice (scale bar = 100 μm). B The liver weight ratio to body of mice, serum ALT, TG and TC levels in CD-fed mice and EtOH-fed mice. C, D Western blot and qRT-PCR analysis of Pparα and Srebp1c in CD-fed mice and EtOH-fed mice. E Western blot analysis of Rev-erbα in CD-fed mice and EtOH-fed mice. F qRT-PCR analysis of Rev-erbα and Rev-erbβ in CD-fed mice and EtOH-fed mice. G HIC shows the expression of Rev-erbα in CD-fed mice and EtOH-fed mice (scale bar = 100 μm or 20 μm) (n = 6). Bar represents the mean ± SEM. Significance *P < 0.05, **P < 0.01 vs. CD-fed group. ^#P < 0.05, ^##P < 0.01 vs. Rev-erbα in CD-fed group