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Fig. 4 | Cell & Bioscience

Fig. 4

From: A ribonucleoprotein transfection strategy for CRISPR/Cas9‐mediated gene editing and single cell cloning in rainbow trout cells

Fig. 4

cyp1a1_mutA and cyp1a1_mutB clonality assessment. Upper and lower panels show the sequencing results obtained from 16 colonies following cloning of the cyp1a1 gene from cyp1a1_mutA and cyp1a1_mutB, respectively, into pBluescript KS+. The 20 nucleotide sequences targeted by the crRNA is highlighted in grey, PAM sequence is highlighted in black, inserted sequences are highlighted in pink. All sequenced plasmids showed the expected genetic mutation in the cyp1a1 region targeted by the crRNA

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Cell & Bioscience

ISSN: 2045-3701

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