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Fig. 3 | Cell & Bioscience

Fig. 3

From: A ribonucleoprotein transfection strategy for CRISPR/Cas9‐mediated gene editing and single cell cloning in rainbow trout cells

Fig. 3

Isolation of RTgutGC clonal cell lines and sequencing results CRISPR/Cas9-gene edited cells. a Micrographs of RTgutGC colonies taken after 5, 14 and 21 days post low density seeding. b cyp1a1 sequencing results of cyp1a1_mutA (upper panel) and cyp1a1_mutB (lower panel) gene-edited clones indicating 1 bp and 101 insertions, respectively. The gRNA position within cyp1a1 gene sequence is schematically indicated below each sequencing result. c Schematic representation of the homology between the 101 bp insertion (blue rectangle) found in the targeted region of cyp1a1 in the cyp1a1_mutB mutant cell line and the closely related gene cyp1a3 (dashed blue rectangles)

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