Fig. 5

The dysfunction of macrophages in peritoneal fluid of endometriosis. a GSEA of Fc gamma r mediated phagocytosis pathway in different clusters of macrophages in endometriosis and control samples. b CD14+ macrophages phagocytosis was detected by flow cytometry in endometriosis and control groups. Light red and open red histograms correspond to background and fluorescence intensity of CD14+ macrophages with ingested bioparticles. Bar plot shows the mean fluorescence intensity of CD14+ macrophages in endometriosis group (n = 5) and control group (n = 5), P = 0.0200. Data are presented as mean ± SEM. c GSEA of the corresponding KEGG pathways in different clusters of macrophages in endometriosis and control groups. d The levels of mRNA expression for VEGF, TGFB1, HLA-DQA and SPPI of primary macrophages in peritoneal fluid from the endometriosis group (n = 10) and control group (n = 9). Data in d are presented as mean ± SEM. EMs endometriosis, NES normalized enrichment score, FDR false discovery rate, GSEA Gene Set Enrichment Analysis