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Fig. 4 | Cell & Bioscience

Fig. 4

From: Cell subtypes and immune dysfunction in peritoneal fluid of endometriosis revealed by single-cell RNA-sequencing

Fig. 4

Two newly discovered subtypes of macrophages in peritoneal fluid. a Double immunofluorescence staining for CD14 (red) and TCR Cβ1 (green) antigens of ascites cell. Original magnification 1000×, scale bars = 10 µm. b The rate of TCR Cβ1 positive cells in isolated CD14 positive peritoneal macrophages tested by flow cytometric analysis. Bar plot shows the percentage of TCR Cβ1 positive macrophages in CD14 positive peritoneal macrophages isolated from the endometriosis (n = 13) and control samples (n = 9). c Double immunofluorescence staining for CD14 (red) and KI67 (green) antigens of ascites cell. Original magnification 1000×, scale bars = 10 µm. d The rate of KI67 positive cells in isolated CD14 positive peritoneal macrophages was tested by flow cytometric analysis. Bar plot shows the percentage of KI67 positive macrophages in CD14 positive peritoneal macrophages isolated from the endometriosis (n = 11) and control samples (n = 9). Data in b and d are presented as mean ± SEM. EMs endometriosis

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