Fig. 1

Cell profiling in peritoneal fluid delineated by single cell transcriptomic analysis. a The workflow that depicts the collection and processing of peritoneal fluid from endometriosis and control patients for single-cell RNA-sequencing and further study. b The heatmap exhibited that the differentially expressed genes of 17,530 isolated cells gathered in 19 clusters. c Co-expressions of PTPRC/CD45, CD68, CD14 and FCGR3A/CD16 (macrophage makers) on the UMAP plot. d Co-expressions of KLRB1, ITGAX and CD1C on the UMAP plot. e 2D visualization of ten cell types on the UMAP plot. Each dot corresponds to one single cell. f Violin plots of specific marker genes in all cell types. g Bar plot of proportions of all cell types in the endometriosis and control samples. EMs endometriosis, NK cells natural killer cells, NKDCs natural killer dendritic cells