Fig. 9

Co-localization of the mouse PRAMEL1 and DDX4 or KIF17b in germ granules of spermatogenic cells by IEM. Both PRAMEL1 (red arrows, 10 nm gold particles) and DDX4 (red arrowheads, 5 nm gold particles) immunogold particles were detected in the IMC (A, B) and CB region (C). The clusters of PRAMEL1 and DDX4 labeling could be overlapped (C), but not always correlated with each other (A, B). In contrast, PRAMEL1 (red arrows, 10 nm gold particles) and KIF17b (red arrowheads, 5 nm gold particles) labeling was colocalized in the same region of the IMC (D, E) and CB (F). Inserts enlarge the boxed regions (C–F). Unlabeled bar = 0.25 µm