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Fig. 5 | Cell & Bioscience

Fig. 5

From: Subcellular localization of the mouse PRAMEL1 and PRAMEX1 reveals multifaceted roles in the nucleus and cytoplasm of germ cells during spermatogenesis

Fig. 5

Localization of PRAMEL1 in CB of spermatids. Large clusters of PRAMEL1 gold particles were detected in the early spermatid (step 1) when CB was formed (A). As CB continues growing in step 3–4 spermatids (BE), the PRAMEL1 protein was enriched and spread over the entire CB. The size of CB decreases when the spermatid begins to elongate (step 5). The image in F showed a step 9 spermatid, in which PRAMEL1 particles were clearly seen in the nascent flagellum and the nearby CB. Mitochondria-associated granule (MAG) or late CB was present in the cytoplasm droplet (CD) of elongated spermatids (step14) (G). The enlarged image in G indicated the enriched PRAMEL1 labeling in the late CB and the associated mitochondria. The gold particles were hardly detected in the nucleus and CB in the negative control (H). M: mitochondria; N: nucleus; NE: Nuclear envelope. Scale bar = 0.5 µm

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