Fig. 1

Localization of the mouse PRAMEL1 protein in developing spermatids and spermatozoa. Smash preparations of seminiferous tubules (A–G and J) were stained with anti-PRAMEL1 (green), anti-β-TUBULIN (red) antibody and DAPI (blue). Spermatozoa (H and I) were stained with anti-PRAMEL1 (green) and DAPI (blue). Merged images for the triple- staining in round spermatids (A and B), elongating spermatids (C–F) and elongated spermatids (G) were shown. PRAMEL1 staining was observed in acrosomal vesicle region of all the developing spermatids and β-TUBULIN labeling was observed in manchette of elongating and elongated spermatids. PRAMEL1 staining was observed in acrosome and midpiece of sperm tail in mature spermatozoa (H). As controls, spermatozoa (I) and smashed tubules (J) were stained with secondary antibody (FITC-labeled donkey anti-goat IgG and TRITC-labeled donkey anti-mouse IgG) and DAPI. No PRAMEL1-specific staining was observed in the controls. Magnification is × 1000. Scale bar = 10 µm