Fig. 6

RDS inhibits influenza A virus infection of MDCK cells. A MDCK cells were pre-treated with serially diluted RDS for 30 min, and then infected with FluA(GFP) virus. Following infection, cells were cultured in the presence of RDS. Inhibition of viral infection was quantified at 36 h post infection with flow cytometry. Uninfected cell and FluA(GFP)-infected but RDS-untreated cells were used as controls. The percentages of GFP + cells are shown. PI, propidium iodide. B The cytotoxicity of RDS on MDCK cells was also quantified using MTT assay. The dose–response cytotoxicity curve was plotted, and the LC₅₀ of RDS was calculated to be at 1:18.5 dilution