Fig. 6

CpG-ODN and BUD synergistically affect HDAC2, RORγt and IL-17 A in HBE cells. Representative micrographs of immunofluorescent staining of HBE cells for detecting tubulin GFP(green; HDAC2-positive) and H2BmCherry(Red; RORγt-positive) cells (×200) (a). HDAC2-positivity rate was determined as tubulin GFP(green)-positive area/total area (b). RORγt-positivity rate was evaluated as H2BmCherry(Red)-positive area/total area (c). Representative images depicting IL-17 A⁺, HDAC2⁺, and RORγt⁺ cells in HBE cell populations (d). The ratios of specific protein-positive HBE cells to total HBE cells (e-g) were assessed by flow cytometry. Statistical significance denoted:^{*, #}p < 0.05, ^**,##p < 0.01. ^* versus the vehicle control group; ^# versus the indicated group