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Fig. 3 | Cell & Bioscience

Fig. 3

From: CpG oligodeoxynucleotides attenuate RORγt-mediated Th17 response by restoring histone deacetylase-2 in cigarette smoke-exposure asthma

Fig. 3

CpG-ODN and BUD synergistically affect HDAC2 activity and expression in chronic CS-exposed asthmatic mice. Representative photomicrographs of immunohistochemical staining of lung sections for HDAC2 in various animal groups (×200) (a). HDAC2-positivity rate was determined as HDAC2-positive area/total bronchiole area (b). Immunoblot was carried out for assessing HDAC2 protein amounts in lung tissue specimens, with GAPDH as a loading control; HDAC2/GAPDH ratios were assessed (c, d). The mRNA expression levels of HDAC2 were evaluated by qRT-PCR in lung tissue samples from mice (e). HDAC2 activity was determined with the colorimetric EpiQuik HDAC2 Activity Assay Kit (f) .Statistical significance denoted:*, #p < 0.05, **,##p < 0.01. * versus the vehicle control group; # versus the indicated group

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Cell & Bioscience

ISSN: 2045-3701

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