Fig. 2

CpG-ODN and BUD synergistically alter Th17 responses in the lower airway. RORγt⁺CD4⁺ cells representing Th17 cells were assessed flow-cytometrically (a) and positive cells were quantitated (b). Th17-related cytokine amounts in serum (e) and BALF (f) were determinedby ELISA, and relative mRNA expression was measured by qRT-PCR in lung tissues from mice (c). Immunoblot was carried out for assessing the protein amounts of Th17-associated cytokinesinlung tissues, with GAPDH as a loading control (d); HDAC2/GAPDH ratios were assessed (g). Statistical significance denoted:^{*, #}p < 0.05, ^**,##p < 0.01. *versus the vehicle control group; # versus the indicated group