Table 1 Primer sequences used for RT-qPCR
Gene | Forward (5’ -3’) | Reverse (5’ -3’ ) |
|---|---|---|
Sema3A | TGGGATTGCCTGTCTTTT | GGCCAAGCCATTAAAAGTGA |
Nrp1 | GACAAATGTGGCGGGACCATA | TGGATTAGCCATTCACACTTCTC |
PlexinA | GAATGCAAATGGGCTGGAAAAG | GATAGCGAAGTCCCGTCCCA |
CD86 | ACTTACGGAAGCACCCACG | CTTTGTAAATGGGCACGGC |
IL-1-β | ACGGACCCCAAAAGATGAAG | AGGCCACAGGTATTTTGTCG |
IL-6 | ATACCACTCCCAACAGACC | TGCATCATCGTTGTTCATAC |
iNOS | GCCACGGACGAGACGGATAG | GGGCACATGCAAGGAAGGG |
TNF-α | CCTCTTCTCATTCCTGCTTG | TCTGGGCCATAGAACTGATG |
CD206 | CCACGGATGACCTGTGCTC | GGTTCCACACCAGAGCCATC |
Arg1 | CAACACTCCCCTGACAACC | ACGATGTCTTTGGCAGATATG |
IL-10 | AGCCGGGAAGACAATAACTG | GGAGTCGGTTAGCAGTATGTTG |
Ym1 | TGCGTGACTATGAAGCATTG | TGACGGTTCTGAGGAGTAGAG |
Actin | TTCTACAATGAGCTGCGTGTG | CCATCACAATGCCTGTGGT |