Fig. 5

Increased M1-like and decreased M2-like phenotypes observed in primary microglia treated with Sema3A. a mRNA expression of cytokines secreted by primary microglia treated with Sema3A. Gene expressions were normalized to β-actin. The results are representative of three independent experiments and are shown as mean ± SEM (n = 3). b, c Representative confocal images and quantitative analysis showing immunofluorescent staining of CD16/32 (red, white arrow) and CD206 (green, white arrow) with Hoechst (blue) nuclear staining in primary microglia 1 day and 3 days after Sema3A and LPS treatment. Scale bar = 100 μm