Fig. 3

At 7 days post-ONC, activated microglia show significantly increased M1-like phenotype and decreased in M2-like phenotype. a Expression of CD16/32 and CD206 in the retina at 3 and 7 days post-ONC. b, c Quantitative analysis indicates a significant increase of CD16/32and CD206 at 3 days post-injury. Level of CD16/32 continuous to increase at 7 days post-ONC, while CD206 decreased significantly. (Mean ± SEM, n = 12). d Representative confocal images showing immunofluorescent staining of CD16/32 (red), CD206 (green) and Iba1 (white) with Hoechst (blue) nuclear staining in retina cells of sham, ONC3D, and ONC7D groups. Scale bar = 25 μm. Quantitative analysis indicates a significant increase of CD206 at 3 days post-injury, and an increase of CD32 both at 3 days and 7days post-injury. Ratio of the number of double positive CD32/Iba1 cells vs. Iba1 positive cells (bar graph). Ratio of the number of double positive CD206/Iba1 cells vs. Iba1 positive cells (bar graph). e mRNA expression of cytokines secreted by microglia in retina at 7 days post-ONC measured by RT-qPCR. Gene expressions were normalized to β-actin. The results are representative of three independent experiments and are shown as mean ± SEM (n = 3)