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Fig. 2 | Cell & Bioscience

Fig. 2

From: Semaphorin3A increases M1-like microglia and retinal ganglion cell apoptosis after optic nerve injury

Fig. 2

Sema3A is significantly reduced in microglia depleted mice retina. a Representative confocal images showing immunostaining of Iba1 (red arrows) with Hoechst nuclear staining. Scale bar = 250 μm. b Quantification of positive Iba1 in sham and microglia depletion groups (n = 4). Iba1 staining was reduced after PLX-3397 treatment. c, d Western Blot shows the expression levels of Sema3A and Iba1 in retina after microglia depletion (MD). β-actin was used as a loading control. Quantitative analysis indicates a significant down-regulation of Sema3A in MD group (Mean ± SEM, n = 5). e Representative confocal images showing immunostaining of Iba1. Scale bar = 25 μm. f Representative confocal images showing immunostaining of Sema3A (green) and Iba1(red) in retinal cryosections. Scale bar = 25 μm

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