Skip to main content
Fig. 1 | Cell & Bioscience

Fig. 1

From: Semaphorin3A increases M1-like microglia and retinal ganglion cell apoptosis after optic nerve injury

Fig. 1

Sema3A is increased in ONC mice retina and is accompanied by microglia activation. a Schematic diagram of the ONC model demonstrating the site of ONC. b Western blot of Sema3A and Iba1 expression in retina at 3 and 7 days (3D and 7D) after ONC. β-actin was used as a loading control. c, d Quantitative western blot analysis indicates a significant up-regulation of Sema3A and Iba1 (Mean ± SEM, n = 10). e, f mRNA expression of Sema3A and its receptors in retina at 3 and 7 days post-ONC as measured by quantitative RT-qPCR. Gene expressions were normalized to β-actin. The results are representative of ten independent experiments and are shown as mean ± SEM (n = 10) (G) Representative confocal images showing immunostaining of Sema3A and Iba1 in retinal cryosections. Sema3A was mainly detected in the ganglion cell layer (GCL) and Iba1 was mainly distributed in GCL, IPL and OPL. Scale bar = 250 μm

Back to article page