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Fig. 8 | Cell & Bioscience

Fig. 8

From: Blocking the interaction between interleukin-17A and endoplasmic reticulum stress in macrophage attenuates retinal neovascularization in oxygen-induced retinopathy

Fig. 8

Effects of MCC950 on the production of IL-17A under hypoxia conditions both in vivo and in vitro. a Representative immunoblots and densitometric analyses of TXNIP, NLRP3, IL-1β and IL-17A protein expression levels in OIR retinas treated with different concentrations of MCC950 (0, 0.01, 0.1, 1, 10 mM). b Representative immunoblots and densitometric analyses of TXNIP, NLRP3, IL-1β and IL-17A protein expression levels in macrophages cultured under hypoxic conditions treated with different concentrations of MCC950 (0, 0.01, 0.1, 1, 10 μM). c Representative immunoblots and densitometric analyses showed alterations of TXNIP, NLRP3, IL-1β and IL-17A protein expression levels in macrophages cultured under hypoxic conditions following TXNIP gene silenced with TXNIP shRNA. β-actin served as an endogenous reference for normalization. Data are shown as mean ± SEM, n = 6–8 per group for Real-time RT-PCR, n = 3 per group for western blotting. Each experiment repeated three times. ns, no significance. * P < 0.05 and **P < 0.01 compared with control groups or each other

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