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Fig. 5 | Cell & Bioscience

Fig. 5

From: Blocking the interaction between interleukin-17A and endoplasmic reticulum stress in macrophage attenuates retinal neovascularization in oxygen-induced retinopathy

Fig. 5

Effects of IL-17ANab or 4-PBA on ER stress markers and IL-17A production in OIR retinas. a Western blotting analysis of GRP78, ATF-4, CHOP and IL-17A protein levels in normal retinas and OIR retinas pretreated with 4-PBA (10 nmol/μl) or IL-17ANab (1.0 μg/μl) or vehicle. b–d Immunofluorescent staining of F4/80 (red) and ATF-4 (b, green), GRP78 (c, green), IL-17A (d, green) in OIR retinas treated with 4-PBA or IL-17NAab or vehicle. Boxed areas are magnified in the images. Yellow represents co-localization of ATF-4 or GRP78 and macrophages infltration. Scale bars, 100 μm. β-actin served as an endogenous reference for normalization. Data are shown as mean ± SEM, n = 6–8 per group for Real-time RT-PCR, n = 3 per group for western blotting. Each experiment repeated three times. * P < 0.05 and **P < 0.01 compared with control groups or each other. ONL, outer nuclear layer; OPL, outer plexiform layer; INL, inner nuclear layer; IPL, inner plexiform layer; GCL, ganglion cell layer

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