Fig. 3

Neutralization or knockout of IL-17A dampened ER stress in macrophages cultured under hypoxic conditions. a, d Real-time RT-PCR and western blotting analysis of GRP78, ATF-4 and CHOP mRNA (a) and protein (d) levels in macrophages pretreated with rmIL-17A for 24 h at different concentrations (0, 5, 10, 25, 50, 100 ng/ml). b, e Real-time RT-PCR and western blotting analysis of GRP78, ATF-4, CHOP and IL17A mRNA (b) and protein (e) levels in macrophages exposed to hypoxia for 12 h pretreated with IL-17ANab at different concentrations (0, 0.5, 1.0 μg/ml). c Changes of GRP78, ATF-4 and CHOP mRNA levels in macrophages from WT mice and IL-17A KO mice cultured under hypoxic conditions for 12 h. β-actin served as an endogenous reference for normalization. Data are shown as mean ± SEM, n = 6–8 per group for Real-time RT-PCR, n = 3 per group for western blotting. Each experiment repeated three times. ns, no significance. * P < 0.05 and **P < 0.01 compared with control groups