Skip to main content
Fig. 2 | Cell & Bioscience

Fig. 2

From: Blocking the interaction between interleukin-17A and endoplasmic reticulum stress in macrophage attenuates retinal neovascularization in oxygen-induced retinopathy

Fig. 2

Neutralization or knockout of IL-17A inhibited ER stress in OIR retinas. a Effects of rmIL-17A (100 ng/μl) stimulation on the protein levels of GRP78, ATF-4 and CHOP in retinas from WT normal mice. b Effects of different concentrations of IL-17ANab (0, 0.5, 1.0 μg/μl) on GRP78, ATF-4 and IL-17A protein levels in retinas from WT-OIR mice. c Immunofluorescent staining of F4/80 (red) and ATF-4 (green), GRP78 (green) on representative sections of retinas from normal WT mice with PBS or rmIL-17A stimulation. d Immunofluorescent staining of F4/80 (red) and ATF-4 (green), GRP78 (green) in retinas of OIR mice from WT ones and IL-17A KO ones. Boxed areas are magnified in the images. Yellow represents co-localization of GRP78 or ATF-4 and macrophages infltration. Scale bars, 100 μm. e, f Changes of retinal GRP78, ATF-4 and CHOP protein (e) and mRNA (f) levels in OIR mice from WT ones and IL-17A KO ones. β-actin served as an endogenous reference for normalization. Data are shown as mean ± SEM, n = 6–8 per group for Real-time RT-PCR, n = 3 per group for western blotting. Each experiment repeated three times. ns, no significance. * P < 0.05 and **P < 0.01 compared with control groups, ##P < 0.01 compared with each other. ONL, outer nuclear layer; OPL, outer plexiform layer; INL, inner nuclear layer; IPL, inner plexiform layer; GCL, ganglion cell layer

Back to article page