Fig. 7
From: Ameliorative role of SIRT1 in peritoneal fibrosis: an in vivo and in vitro study
SIRT1 was downregulated in TGF‐β stimulated Met‐5A cells and Adenovirus transfection of SIRT1. a SIRT1 mRNA expression assessed by qRT‐PCR in Met‐5A cells exposed to control conditions (unstimulated) or TGF‐β1 for 48 h. b SIRT1 protein level assessed by Western blotting under identical conditions and the relative expressions were normalized to control. c Met‐5A cells were transfected with Ad‐sh‐SIRT1, or Ad‐SIRT1 for 48 h. Non-transfected cells were included as control. SIRT1 mRNA expression assessed by qRT‐PCR. (D) SIRT1 protein level assessed by Western blotting after transfection and the relative expressions were normalized to control. Data are presented as mean ± SD. n = 4 for each group. *p < 0.05, **p < 0.01, ***p < 0.001 between the indicated groups