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Table 1 Domain structure function relationship

From: Physiological relevance of post-translational regulation of the spindle assembly checkpoint protein BubR1

Domain Function
KEN1 APC/C degron; Binds Cdc20
MCC assembly: Brings together BubR1, Bub3, Mad2, Cdc20
TPR motifs Binds KNL1 protein (MELT motifs) on kinetochores
Anaphase inhibition: Binds an E2 binding domain on APC/C
D box 1 APC/C degron; Binds Cdc20 at its D box recognition domain, preventing recognition of D box-containing substrates
ABBA motif (A1) Binds Cdc20
KEN2 APC/C degron; Binds Cdc20
APC/C inhibition: Blocks substrate recruitment to APC/C
ABBA motif (A2) Binds Cdc20
GLEBS;
Bub3-binding
BubR1 recruitment to kinetochores via Bub3 binding
ABBA motif (A3)
(Phe box; IC20BD)
Binds Cdc20-APC/C
Unknown the extent of its importance
D box 2 APC/C degron; Binds Cdc20 at its D box recognition domain, preventing recognition of D box-containing substrates
KARD PP2A-B56 recruitment to kinetochores
Kinase domain Potentially catalytic: autophosphorylation, targeted phosphorylation of other mitotic proteins
May be involved in BubR1 protein stability