Domain | Function |
---|---|
KEN1 | APC/C degron; Binds Cdc20 MCC assembly: Brings together BubR1, Bub3, Mad2, Cdc20 |
TPR motifs | Binds KNL1 protein (MELT motifs) on kinetochores Anaphase inhibition: Binds an E2 binding domain on APC/C |
D box 1 | APC/C degron; Binds Cdc20 at its D box recognition domain, preventing recognition of D box-containing substrates |
ABBA motif (A1) | Binds Cdc20 |
KEN2 | APC/C degron; Binds Cdc20 APC/C inhibition: Blocks substrate recruitment to APC/C |
ABBA motif (A2) | Binds Cdc20 |
GLEBS; Bub3-binding | BubR1 recruitment to kinetochores via Bub3 binding |
ABBA motif (A3) (Phe box; IC20BD) | Binds Cdc20-APC/C Unknown the extent of its importance |
D box 2 | APC/C degron; Binds Cdc20 at its D box recognition domain, preventing recognition of D box-containing substrates |
KARD | PP2A-B56 recruitment to kinetochores |
Kinase domain | Potentially catalytic: autophosphorylation, targeted phosphorylation of other mitotic proteins May be involved in BubR1 protein stability |