Fig. 9

HBV induced changes in the regulation Host heterogeneous nuclear ribonucleoproteins (hnRNPs), E3s and DUBs. a L/H ratios for selected regulated proteins from three biological replicates in HepG2.2.15 cells. b The levels of the secreted HBeAg and HBsAg were determined by ELISA and HBV total RNA by RT-PCR from samples co-transfected (1:1) with pHBV1.3 and indicated plasmids. c L/H ratios for selected regulated proteins from three biological replicates in HepG2.2.15 cells. d L/H ratios for selected regulated Kub-sites & proteins from three biological replicates in HepG2.2.15 cells. e, f Co-immunoprecipitation and immunoblot analysis of extracts of HepG2.2.15 & HepG2 cells transfected with plasmid encoding Flag-OTULIN, Flag-OTULIN-K66-R and Hepatitis B virus (HBV) 1.3-fold genome plasmid (pHBV1.3) and treated with MG132, are shown