Fig. 6

Motif analysis of all the identified Kub sites and protein–protein interaction of ubiquitinated proteins in HepG2.2.15 cells. a, b L/H ratios for selected regulated Kub-sites & proteins from three biological replicates in HepG2.2.15 cells. c Associations among proteins with regulated Kub-sites based on STRING and visualized in Cytoscope. d Ubiquitination motifs and the conservation of Kub-sites. The height of each letter corresponds to the frequency of that amino acid residue in that position. The central K refers to the ubiquitinated Lys. e Number of identified peptides containing ubiquitinated Lys in each motif. f Amino acid sequence properties of ubiquitylation sites. The heat map shows significant position-specific underrepresentation or overrepresentation of amino acids flanking the modification sites