Fig. 5From: RRM2 silencing suppresses malignant phenotype and enhances radiosensitivity via activating cGAS/STING signaling pathway in lung adenocarcinomaRRM2 silencing activated cGAS/STING signaling pathway. a, b Immunostaining was performed to determine the accumulation of dsDNA in cytoplasm. The representative images were taken with confocal microscope. Scale bar: 10 μm. c The numbers of dsDNA spots were quantified and graphed. d The correlation analysis between the expression of RRM2 and STING in LUAD patients was performed with TIMER database. e Immunoblotting of the classical cGAS/STING pathway proteins including cGAS, p-STING, STING, p-IRF3 and IRF3. GAPDH was used as an internal control. f The mRNA levels of STING were detected with qRT-PCR in A549 and PC9 cells after RRM2 silencing. g The mRNA levels of cGAS/STING downstream molecules (IFNβ, CCL5 and CXCL10) were detected by qRT-PCR in RRM2-deficient cells. h The secretion of IFNβ, CCL5 and CXCL10 were assayed by ELISA in RRM2-deficient cells. All tests were repeated 3 times. **p < 0.01, ***p < 0.001Back to article page