Fig. 6

KLF7/VPS35 axis contributed to HCC progression via enhancing the β-catenin signaling. a Knockdown of KLF7 and VPS35 affected active-β-catenin and GS expression in HCC cells detected by western blot, respectively. b Overexpression of KLF7 and VPS35 affected active-β-catenin expression in HCC cells detected by western blot, respectively. c KLF7-silencing and VPS35-overexpressing affected active-β-catenin expression in Huh-7 and SKHEP1 cells. d KLF7-overexpressing and VPS35-silencing affected active-β-catenin level in Huh-7 and SKHEP1 cells. e The correlation between VPS35 and β-catenin expression in HCC and normal tissues. f, g Cell viability of VPS35-overexpressing HCC cells treated with GK974 in Huh-7 and SKHEP1 cells. h Representative images of colony formation assay in VPS35-overexpressing HCC cells treated with GK974. i, j Colony formation of VPS35-overexpressing cells treated with GK974 in Huh-7 and SKHEP1 cells