Fig. 5

KLF7 contributes to tumor progression by regulating VPS35 expression. a Western blot analysis of VPS35 and cyclin D1 in si-KLF7 and siCtrl HCC cells. b A relationship between VPS35 and KLF7 was identified by TCGA database. d Luciferase reporter assay was used to determine the luciferase activity in HCC cells transfected with empty plasmids or plasmids containing VPS35. e ChIP-qPCR analysis of the interaction between KLF7 and VPS35 promoter sequence. f Western blot analysis of VPS35 in VPS35 knockdown HCC cells. g Cell viability of VPS35 knockdown HCC cells determined by CCK-8 assay. h Western blot analysis of VPS35 in KLF7 overexpression and VPS35 knockdown HCC cells. i Cell viability of KLF7 overexpression and VPS35 knockdown HCC cells determined by CCK-8 assay. j, k Representative images and statistical data of colony formation assay after simultaneously transfected with KLF7-overexpressing and VPS35-silencing SKHEP1 cells. l, m Representative images and statistical data of cell invasion assay after simultaneously transfected with KLF7-overexpressing and VPS35-silencing SKHEP1 cells. **p < 0.01