Fig. 2

Downregulation of KLF7 inhibited HCC cell proliferation, invasion and induced cell cycle arrest and apoptosis. a Western blot assay examined knockdown of KLF7 expression mediated by siRNA in two HCC cell lines (Huh-7 and SKHEP1). b, c CCK-8 assay was performed to determine the proliferation capacity of KLF7-silencing Huh-7 (b) and SKHEP1 cells (c) after transfection with siRNA or siCtrl. d, e Colony formation assay was applied to measure the proliferation of si-KLF7-transfected Huh-7 and SKHEP1 cells. f Flow cytometry assay was used to detect cell apoptosis rate in Huh-7 and SKHEP1 cells. g, h Flow cytometry assay was conducted to identify the stages of cell cycle progression in Huh-7 and SKHEP1 cells. i, j Transwell assay was used to examine cell invasion in Huh-7 and SKHEP1 cells transfected with si-KLF7 and siCtrl. Each experiment was independently repeated at three times. *p < 0.05, **p < 0.01