Fig. 6
From: Myelin basic protein enhances axonal regeneration from neural progenitor cells
Stable Mbp overexpression in scaffolded mNPCs produces superior locomotive recovery and axonal regeneration in a murine model of SCI. a Experimental schematic overview of murine model of SCI detailing timepoints for spinal cord crush injury (SCI), grafting, locomotive fault analysis, and sacrifice for histological analysis. b H&E-stained parasagittal spinal cord sections 6 weeks post-SCI. Scale bar, 200 μm. c Quantification of fault rates by horizontal ladder walking assay post-SCI. d Boxplots of fault rates at 9, 10, 11, and 12 weeks post-SCI. e Representative immunofluorescent images of parasagittal spinal cord sections 6 weeks post-SCI stained for bovine collagen I, neurofilament heavy chain (NF), and L1-70. Scale bar, 200 μm. f Quantification of L1-70 + pixel density 9 weeks and 12 weeks post-SCI caudally, epicentrally, and rostrally to the SCI lesion. Results are normalized to the Sham Ctrl group. Panels report either means ± standard deviations (SDs) [bar and line charts] or medians ± interquartile ranges (IQRs) [boxplots]. n = 9 animals per cohort. *P < 0.05, **P < 0.01 [one-way ANOVA, post-hoc Tukey’s test]