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Fig. 5 | Cell & Bioscience

Fig. 5

From: Myelin basic protein enhances axonal regeneration from neural progenitor cells

Fig. 5

Mbp/L1-70 axis regulates expression of PPARγ’s dendrite regeneration-associated gene targets in NPCs. Wild-type (WT) and Mbp-null (Mbp−/−) spinal cord-derived E12 mouse neural progenitor cells (E12 mNPCs) transduced with pcDNA3-Ctrl or pcDNA3-VP16-PPARγ2 and treated with the PPARγ agonist ciglitazone (1 µM) after 48 h of Poly-d-lysine control (PDL Ctrl) or myelin (Mye) substrate culture. WT PDL Ctrl was used to normalize values. a–c PCR quantification of anti-PPARγ immunoprecipitated chromatin using site-specific primers. d–f qPCR of PPARγ target gene expression. g, h Activation of extracellular signal–regulated kinase 1/2 (Erk1/2) signaling assayed by g ELISA and h immunoblotting. All panels report means ± standard deviations (SDs). n = 3 embryos/genotype × 3 wells/embryo. *P < 0.05, **P < 0.01 [two-way ANOVA, post-hoc Tukey’s test]

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